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rimM [2020-04-06 11:02:21]
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rimM [2020-04-06 11:02:21]

16S rRNA processing protein, RNase
Locus
BSU_16020
Isoelectric point
5.22
Molecular weight
20.06 kDa
Protein length
174 aa Sequence Blast
Gene length
525 bp Sequence Blast
Function
maturation of 16S rRNA
Product
16S rRNA processing protein, RNase
Essential
no
Synonyms
ylqE

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,674,650 1,675,174

The protein

Protein family

  • RimM family (single member, according to UniProt)
  • Domains

  • PRC barrel domain (aa 98-171) (according to UniProt)
  • Structure

  • 3H9N (from Haemophilus influenzae, 33% identity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulation

  • the mRNA is processed between ffh and rpsP by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
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    Biological materials

    Mutant

  • MGNA-B376 (ylqE::erm), available at the NBRP B. subtilis, Japan
  • BKE16020 (rimM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGATCACCTCTGGCGA, downstream forward: _UP4_GAATGAAAATCGATTTTTTG
  • BKK16020 (rimM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGATCACCTCTGGCGA, downstream forward: _UP4_GAATGAAAATCGATTTTTTG
  • References

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